The impact of SLC10A3 on prognosis and immune microenvironment in colorectal adenocarcinoma

Background SLC10A3, a gene upregulated in pan-cancer, lacks full understanding regarding its prognostic implications and association with immune infiltration in colorectal cancer (CRC). This study comprehensively analyzed SLC10A3 in CRC, evaluating its prognostic significance and influence on the tumor's immune microenvironment. Methods Transcriptomic data from TCGA were obtained to compare SLC10A3 expression in both colorectal cancer (CRC) and normal tissues. Prognostic value was assessed for overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI). DNA methylation patterns of SLC10A3 and correlation with DNA mismatch repair (MMR) were explored. Genetic alterations in SLC10A3 were scrutinized. The study also delved into the influence of SLC10A3 on the immune microenvironment of CRC, including immune cell infiltration and chemokines. Involvement of cancer-associated fibroblasts (CAFs) was explored. Methylation status of specific CpG islands in the SLC10A3 gene correlated with CRC patient prognosis. CRC tissue microarray was performed to verify the expression of SLC10A3 and its relationship with prognosis. Results The research revealed that SLC10A3 is significantly upregulated in CRC and holds promise as a potential diagnostic marker. Elevated SLC10A3 expression was linked to poorer OS, DSS, and PFI. Methylation patterns of SLC10A3 displayed prognostic relevance, and genetic alterations in the gene were identified. SLC10A3 was shown to impact the immune microenvironment, with significant correlations observed between its expression and various immune cell types, chemokines, and markers associated with CAFs. Furthermore, an inverse relationship between SLC10A3 and MMR molecules was established. Methylation status of specific CpG islands within the SLC10A3 gene was associated with CRC patient prognosis. Tissue microarray showed that SLC10A3 was highly expressed in CRC and significantly correlated with poor prognosis. Conclusion The study underscores the importance of elevated SLC10A3 in CRC, associating it with decreased survival and immune infiltration, proposing it as a diagnostic biomarker and appealing immunotherapy target, given its significant overexpression and influence on the immune microenvironment and prognosis through methylation patterns. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s40001-023-01526-4.


Introduction
Colon cancer is a major human cancer, ranking the top three cancers in the United States in terms of incidence as well as mortality in 2022 [1].According to the data from 2014, it accounts for about 10% of all cancer cases in China [2,3].Colorectal cancer (CRC), including colon and rectum cancer, is often grouped due to their common characteristics, primarily manifesting as adenocarcinomas [4].CRC can result from a combination of age, lifestyle, and genetic factors [5].The progression from polyps to CRC is multifactorial, involving gene mutations, epigenetic modifications, and local inflammatory changes.Over the past 30 years, extensive research has identified numerous molecular alterations that contribute to the development and advancement of colon polyps.This research has unveiled multiple pathways leading to the heterogeneous presentation of colon polyps and CRC, characterized by global DNA abnormalities, including aneuploidy and microsatellite instability, as well as specific patterns of epigenetic changes (e.g., CpG Island Methylator Phenotype or CIMP), mutations in specific genes, and aberrantly methylated genes [6].Comprehensive transcriptomics confer a precise characterization of individual cancers that should help to improve clinical strategies for neoplastic diseases through the development of novel drugs.In the past decade, numerous gene expression studies on CRC have been reported, aided by advanced technologies such as microarray and RNA-sequencing, as well as public databases such as Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA).For instance, Liang, Li, and Zhao employed GEO datasets to identify a total of 3500 differentially expressed genes (DEGs) in CRC, consisting of 1370 upregulated and 2130 downregulated genes [7].
Pathway enrichment analysis revealed that upregulated DEGs were enriched in the cell cycle and DNA replication, while downregulated DEGs were enriched in drug metabolism.Additionally, Y. Guo, Bao, Ma, and Yang aimed to identify potential key candidate genes and pathways in CRC using four GEO cohorts and discovered 292 shared DEGs (165 upregulated and 127 downregulated) from the four datasets [8].In another study (Wu, Wu, & Jiang, 2017) [9], gene and microRNA (miRNA) expression profiles from GEO datasets were utilized to identify 600 upregulated DEGs, 283 downregulated DEGs, 13 upregulated miRNAs, and 7 downregulated miRNAs [10].
The Solute Carrier (SLC) Family 10 (SLC10) is involved in the influx transport of bile acids, steroidal hormones, specific drugs, and a variety of other substrates, and comprises seven family members, including SLC10A1, SLC10A2, SLC10A3, SLC10A4, SLC10A5, SLC10A6 and SLC10A7.The SLC10 family is identified as the Bile Acid Sodium Symporter (BASS) family in the transporter classification system and belongs to the bile/arsenite/riboflavin transporter (BART) superfamily [11].Bile acids (BA) are complex signaling molecules that modulate glucose, lipid and energy metabolism and are also implicated in the etiology of certain types of cancer [11].Recent studies suggest that SLC10A3 may influence chemotherapy resistance and serve as a promising immune biomarker in liver cancer.Moreover, the expression of SLC10A3 protein is correlated with stromal CD4 T cells, CD20 B cells, macrophages, PDCD1 (PD-1) and CD274 (PD-L1) expression, indicating a potential regulatory role in liver cancer immunotherapy [12].However, whether SLC10A3 plays a similar role in colorectal adenocarcinoma remains unknown.
Currently, updated public databases based on integrative bioinformatics analysis of the Cancer Genome Atlas (TCGA) have significantly enhanced the efficiency of identification of biomarkers and functional genes in cancerous diseases.Therefore, this study evaluates the transcriptional profiles and potential prognostic value of the SLC10A3 by systematical bioinformatics analysis and provides a novel role of SLC10A3 in the prognostic value and immune microenvironment in colorectal adenocarcinoma.

Transcriptome data sources
The mRNA expression data of SLC10A3 were downloaded from the TCGA database (http:// cance rgeno me.nih.gov/ about tcga) for 33 different types of cancer [13].The transcriptome data were then transformed into Log2 form and analyzed using the Wilcoxon rank-sum test to compare the expression levels between tumor and normal tissues.A p-value < 0.05 was considered statistically significant.To evaluate the predictive value of SLC10A3 for COAD and READ, AUC and ROC analysis were conducted.

Survival analysis
The hazard ratio (HR) and 95% confidence intervals were calculated using univariate survival analysis.To assess the survival outcomes of SLC10A3 in COAD and READ, Kaplan-Meier survival analysis was conducted for OS (overall survival), DSS (disease-specific survival), and PFI (progression-free interval).The survival differences were compared using ''survminer'' and ''surviva'' packages.The concordance index (C-index) was used to evaluate the discrimination of the nomograms.
To explore the DNA methylation of SLC10A3 in TCGA, MethSurv7 was utilized.The analysis involved assessing the methylation levels and prognostic values of each CpG site within SLC10A3.Furthermore, the relationship between the CpG methylation status of SLC10A3 and the overall survival (OS) of CRC was evaluated.

Analysis of mutation
For the genetic alteration analysis of SLC10A3, we utilized the cBioPortal (https:// www.cbiop ortal.org/), a comprehensive website offering visualization, analysis, and download options for large-scale cancer genomics datasets [14].Specifically, we examined the alteration frequency, mutation type, and copy number alterations (CNA) of SLC10A3 in COAD and READ.

Immune cells infiltration
We utilized ssGSEA [15] to investigate the relationship between SLC10A3 and 24 types of immune cell infiltration in the immune microenvironment of CRC.Furthermore, we examined the differences in immune cell abundance between the high and low SLC10A3 expression groups.Using the Tumor Immune Estimation Resource (TIMER) [16], we assessed the association between the expression levels of tumor-infiltrating immune cells, including B cells, CD4 + T cells, CD8 + T cells, neutrophils, macrophages, dendritic cells, and cancer-associated fibroblasts (CAFs), and SLC10A3 expression.The Pearson method was used to display the results.Finally, we explored the correlation between somatic copy number alterations (SCNA) of SLC10A3 and the abundance of 6 leukocytes using the 'SCNA module' .

Functional enrichment analysis
We employed the Cluster Profiler R package to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs between the high and low SLC10A3 expression groups.

Statistical analysis
We performed log2 transformation to normalize all data obtained from TCGA.Spearman's or Pearson's test was used to analyze the correlation between two variables.TCGA (https:// portal.gdc.cancer.gov/) COAD and READ data were utilized for these analyses.For survival analysis, we calculated the hazard ratios (HRs) and p-value using either the univariate Cox regression analysis or log-rank test.A p-value < 0.05 was considered statistically significant for all analyses.

High expression of SLC10A3 in colorectal cancer samples
To explore the biological function of SLC10A3 in pancancer, especially CRC, we performed a series of bioinformatics analyses (Fig. 1A).Firstly, we compared the transcriptional levels of SLC10A3 in pan-cancer between tumor and normal samples by applying the TCGA databases.In TCGA data, differential SLC10A3 expression was significant among 16 of the 33 cancer types analyzed, including 12 upregulation and 4 downregulation (Fig. 1B).Moreover, SLC10A3 expression was higher in tumors than in normal tissues in CRC (Fig. 2A).We evaluated the accuracy of a colon cancer risk prediction model based on SLC10A3 using ROC curve analysis and found that the sensitivity was greater than the specificity, with an area under curve (AUC) of 0.745 (Fig. 2B).This indicates good predictive accuracy for the model.Based on the TCGA database and UALCAN, we found SLC10A3 was highly expressed in COAD and READ (Fig. 2CD).Based on the TCGA database, we found the methylation levels of SLC10A3 did not significantly change in colon and rectum cancer tissues than that in the corresponding normal tissues (Fig. 2EF).

Mutation of SLC10A3 in CRC
Gene mutations play a crucial role in the development and progression of cancer.To gain insights into the mutations associated with CRC, we conducted a study on SLC10A3 gene mutations.Our analysis of the cBioPortal database revealed that SLC10A3 gene alterations were detected in CRC patients with amplification (> 0.5%) (Fig. 3A).The genetic alterations in SLC10A3 were primarily categorized into four types: amplification, truncating mutation, inframe mutation, and missense mutation.We further investigated the types, sites, and case numbers of SLC10A3 gene modification using data from DFCI, Cell Reports 2016, and TCGA Firehose Legacy (Fig. 3B-D).

Association of SLC10A3 with clinical stage and prognosis
We examined the association between SLC10A3 expression and clinical stage in CRC, using high and low expression groups.Our analysis revealed positive correlations between SLC10A3 and lymph node invasion (p-value = 0.006), distant metastasis (p-value < 0.001), and patient age (p-value = 0.049) (Table 1).Moreover, we found that the mRNA level of SLC10A3 was higher in M1 stage than in M0 stage (Fig. 4F).In order to investigate the relationship between SLC10A3 expression and patient survival, we employed the Kaplan-Meier (KM) Plotter.Our results demonstrated that higher mRNA levels of SLC10A3 were significantly associated with shorter OS (p-value = 0.037), DSS (p-value = 0.034), and PFI (p-value = 0.008) in COAD patients (Fig. 4A-C).However, we also observed that high expression of SLC10A3 was linked to shorter OS (p-value = 0.022) and DFS (p-value = 0.026) (Fig. 4D-E).To assess survival rates in COAD patients, we constructed a nomogram based on age, gender, TNM stage, tumor stage, and risk score, and found good consistency between predicted and actual survival rates (Fig. 4G-H).

Role of SLC10A3 in CRC immune microenvironment
The tumor microenvironment plays a crucial role in tumor genesis and development.Therefore, investigating the relationship between SLC10A3 and immune cell infiltration is significant.Immune cell infiltration in CRC was determined using ssGSEA for 24 immune cell types, and the relationship between SLC10A3 expression and immune cell infiltration was analyzed using Pearson's correlation coefficient (Table 3).NK cells, Eosinophils, and TReg showed a positive correlation with SLC10A3 expression, while Tcm, Th2 cells, and T helper cells showed a negative association with SLC10A3 (Fig. 5A).The tumor infiltration levels of TReg cells, CD8 + cells, NK cells, and T cells were consistent with the Pearson's correlation analysis results (Fig. 5B-E).Furthermore, we analyzed the relationship between somatic copy number aberrations (CNA) and tumor immune infiltration using TIMER.Firstly, the ''SCNA'' module analysis showed that altered SLC10A3 gene copy numbers in COAD were associated with immune cell infiltration levels of B cells, macrophages, neutrophils, and dendritic cells (Fig. S1).In comparison, SLC10A3 gene copy numbers were only associated with dendritic cell infiltration levels in READ (Fig. S1).Secondly, the ''Gene'' module analysis confirmed that immune infiltration levels of B cells, CD8 + T cells, CD4 + T cells and macrophages were associated with SLC10A3 expression in READ and COAD (Fig. 5FG).Besides, the results of CIBERSORT, EPIC, QUAN-TISEQ, XCELL, and MCPCOUNTER about the relationship between SLC10A3 and immune infiltration are displayed in Table S1.In conclusion, high expression of SLC10A3 could affect the immune microenvironment of READ and COAD by regulating the proportions of immune cell infiltration.
Chemokine networks affect tumor immunity and tumorigenesis by regulating the tumor microenvironment.Therefore, we next examined the correlations between chemokines and SLC10A3 in CRC.The results showed significant (p-value < 0.05) and strong positive correlations between SLC10A3 expression and the following chemokines: CCL15, CCL24, and CXCL14.Additionally, strong negative correlations were observed between SLC10A3 expression and chemokines including CCL2-5/7-8, CXCL5-6 and CXCL8-13 in COAD (Fig. 5H).These results further confirmed that SLC10A3 was significantly correlated with immune infiltrating cells and chemokines in READ and COAD, indicating that SLC10A3 may play a crucial role in the microenvironment of READ and COAD.

Correlation analysis of SLC10A3 and cancer-associated fibroblasts
Cancer-associated fibroblasts (CAFs) create a conducive environment for tumor growth, as they are known to inhibit immune cell function through secretion of cytokines and metabolites, thereby promoting tumorigenesis, invasion, metastasis, and drug resistance.Our analysis of the correlation between SLC10A3 and CAFs using TIMER revealed a significant association in CRC (Fig. 6AC).Specifically, high expression of SLC10A3 was positively correlated with CAF markers such as ACAT2 and VIM in COAD and READ (Fig. 6BD).

Association of SLC10A3 and DNA mismatch repair (MMR)
We analyzed the correlations between SLC10A3 expression and mismatch repair molecules using Spearman's correlation coefficient.Obviously, SCL10A3 expression showed a negative association with PMS1, RAD21, DNA2, MLH3, and MSH4 (Fig. 7A-F).In summary, our

The methylation status of the SLC10A3 is associated with the prognosis of COAD and READ patients
DNA methylation levels in SLC10A3 were analyzed, and the prognostic value of CpG islands in SLC10A3 was assessed using the MetSurv tool.The findings revealed the presence of 10 methylated CpG islands in COAD and 11 methylated CpG islands in READ.Notably, methylation levels of two specific CpG islands, namely cg06266461 and cg09418475, showed significant association with prognosis (p-value < 0.05) in COAD.In contrast, no CpG islands in READ were found to be associated with prognosis (Table 4).

Screening DEGs and functional enrichment analyses based on SLC10A3 single gene analysis
Based on the TCGA database, we conducted SLC10A3 single gene analysis, divided the tumor samples into SLC10A3 low expression group and SLC10A3 high expression group by R (version 4.1.1),and screened differentially expressed genes in volcano plot (Fig. 8A).DEGs, including 127 upregulated and 2492 downregulated genes, were identified between low and high SLC10A3 groups in CRC (Fig. 8A).The potential bio-function of DEGs was enriched in nucleosome, nucleosome assembly and small nuclear ribonucleoprotein complex via GO and KEGG enrichment analysis in CRC (Fig. 8B).SLC10A3 may promote CRC progression by increasing chromatin synthesis.GSEA showed that the SLC10A3-associated DEGs were significantly enriched in nucleosome organization, protein-DNA complex and nucleosome assembly to regulate the progression of CRC (Fig. 8C).

High SLC10A3 expression is strongly associated with CRC prognosis
We investigated the functions of SLC10A3 in CRC by examining the relative levels of SLC10A3 in patient tissues and corresponding normal tissues.72 patient specimens were analyzed, and SLC10A3 was found to be expressed differently, with some specimens having low levels and others having high levels (Fig. 9A-B).Using IHC scores (cut-off = 7), we divided the specimens into high and low expression groups, with a higher proportion of high SLC10A3 expression in tumor tissues (Fig. 9C).Additionally, the amount of SLC10A3 was significantly increased in CRC tissues compared to normal tissues (Fig. 9C).To predict the diagnostic value of SLC10A3 in distinguishing CRC from the mucosa, we used ROC and AUC, which resulted in an AUC value of 0.879 (Fig. 9D).Our analysis indicated that the level of SLC10A3 is correlated with CRC malignancy in tumor relapse and metastasis (Fig. 9E-F), but not in lymph node invasion status and tumor stage (Fig. S2AB).Besides, high SLC10A3 expression predicted low OS (HR = 2.23, p-value = 0.022) and DFS (HR = 2.24, p-value = 0.026) (Fig. 9G-H).Our data suggest that SLC10A3 may serve as a useful diagnostic and prognostic indicator in CRC patients.

Discussion
Colorectal cancer is the third most common cancer worldwide in terms of incidence and ranks fourth in terms of cancer-related mortality [19].Although significant clinical advancements have been made in the treatment of colorectal cancer through medical techniques, surgical interventions, and chemotherapy [20], the current lack of an effective diagnostic method to monitor CRC progression or recurrence remains a challenge.Recent studies have highlighted the pivotal role of SLC10A3 in various cancers, with its mutation being implicated in malignant growth and cancer development.
Specifically, SLC10A3 plays a critical role in regulating the immune response in liver cancer, related with stromal CD4 T cells, CD20 B cells, and macrophage [9].Upregulation of SLC10A3 mRNA is statistically related with unfavorable survival outcomes and immune infiltration among low-grade gliomas [21].Nonetheless, there is currently no literature on SLC10A3 in colorectal cancer.Thus, this study aims to elucidate the expression of SLC10A3, immunocorrelation and its significant role in predicting the prognosis of colorectal cancer, ultimately establishing a genetic risk-scoring model.In our study, through TCGA database we found that SLC10A3 is significantly higher expressed in colorectal cancer, so we further investigated the role of SLC10A3 in colorectal cancer and unveiled its prognostic value, functional enrichment pathways, methylation patterns, tumor immune infiltration, and variance analysis between high and low SLC10A3 expression groups using bioinformatic analysis.Higher SLC10A3 expression was notably associated with worse prognosis, including shorter overall survival (OS, p-value = 0.037, HR = 1.45(1.02-2.05)),disease-free survival (DFS, p-value = 0.034, HR = 1.63(1.04-2.56)),and progression-free survival (PFI, p-value = 0.008, HR = 1.52(1.12-2.06)).Moreover, high SLC10A3 expression may be linked to metastasis in CRC patients.Abnormal methylation of specific genes in cells poses a high risk of cancer, and this aberrant methylation occurs during cell oncogenesis [22,23].The methylation levels of four CpG islands, including cg06266461 and cg09418475, were found to be associated with prognosis (p < 0.05) in COAD.Tissue microarray of CRC confirmed the high expression of SLC10A3 in tumor tissues, which was indicative of poor prognosis, including OS (p-value = 0.022, HR = 2.23 (1.16-4.29)and DFS (p-value = 0.026, HR = 2.24(1.10-4.56)).Our results demonstrate that CRC patients with high SLC10A3 expression experienced shorter survival times and a higher likelihood of disease progression, highlighting SLC10A3 as an independent risk factor for survival.
Mounting evidence indicates the pivotal role of the immune microenvironment in tumorigenesis [24].Tumor immunity closely intertwines with cell growth, and any disruption in the immune microenvironment can expedite cancer progression [25].Computer algorithms analyzing transcriptomic data have gained widespread adoption in assessing the dynamic immune components within the tumor microenvironment (TME) of cancer patients.In this study, we employed the ssGSEA method to determine the extent of immune cell infiltration and the ratio of immune/mesenchymal components in colorectal cancer (CRC) samples sourced from the TCGA database.Our findings confirmed the prognostic biomarker, SLC10A3, as an influential factor in immune  Subsequently, we conducted correlation analyses between chemokines and SLC10A3 in CRC.Spearman's method was utilized to examine the associations between SLC10A3 expression and immune checkpoint molecules.Additionally, we investigated the relationships between SLC10A3 and various chemokines in both COAD and READ.The results revealed statistically significant (p-value < 0.05) and robust positive correlations with the several chemokines: CCL15, CCL24, and CXCL14.Moreover, strong negative correlations were observed between SLC10A3 and chemokines such as CCL2-5/7-8, CXCL5-6 and CXCL8-13 in COAD.Cancer-associated fibroblasts (CAFs) represent a critical component within the tumor microenvironment, exerting a pivotal role in tumor initiation and progression [26,27].Rather than existing in isolation, CAFs interact with neighboring tumor cells, actively promoting their growth, survival, and sustaining their malignant    The mismatch repair system is a safety net in the body that maintains the integrity and stability of genetic material.In DNA mismatch repair (MMR), repair genes can ensure the ''fidelity'' of the replication process during DNA replication [33].Our results showed SCL10A3 expression was negatively associated with PMS1 (p-value < 0.001), RAD21 (p-value = 0.012), DNA2 (p-value < 0.001), MLH3 (p-value < 0.001), and MSH4 (p-value < 0.001).Low expression in mismatch repair genes can lead to shorten or lengthen microsatellite segments.Microsatellite instability (MSI) will result in a high mutant phenotype of the genome, leading to an increased risk of tumor development [34].
However, it is important to acknowledge the limitations of our study.Our analysis heavily relied on tissue samples from the TCGA dataset, with only 72 pairs of clinical samples used for validation.Therefore, it would be beneficial to validate our findings using larger sample cohorts from different medical centers.Additionally, although we found an association between SLC10A3 and the immune microenvironment of colorectal cancer, confirmatory experiments were not conducted.Moving forward, our further plan includes verifying that SLC10A3 promotes CRC proliferation and metastasis through cytological and animal experiments, as well as exploring its mechanism.This aspect represents a potential future direction for our investigation.

Conclusion
Overall, the findings suggested that SLC10A3 high expression is an independent risk biomarker for worse prognosis in patients with CRC and is related to immune infiltration and distant metastasis.Besides, our study may provide new insights into the treatment of CRC and inform further studies of tumor immunity in CRC.However, studies with larger clinical samples and further functional experiments are required to verify our results.

Fig. 1
Fig. 1 Identify SLC10A3 as a novel biomarker in colorectal cancer.A Flowchart of the present research.B SLC10A3 expression in different tumor and tumor-adjacent tissues in the TCGA database.* indicates p-value < 0.05; ** indicates p-value < 0.01; *** indicates p-value < 0.001

Fig. 2
Fig. 2 Comparative analysis of SLC10A3 expression and methylation levels in colorectal cancer (CRC) and normal tissues based on the TCGA database.A SLC10A3 expression was higher in CRC than in normal tissues based on the TCGA database.B The ROC and AUC of CRC is based on the expression of SLC10A3.C-D SLC10A3 expression was higher in COAD and READ than in normal tissues based on the TCGA database.E-F the methylation levels of SLC10A3 in COAD, READ and normal tissues.*indicates p-value < 0.05; ** indicates p-value < 0.01; *** indicates p-value < 0.001.CRC colorectal cancer, READ rectal adenocarcinoma; COAD colon adenocarcinoma

Fig. 4
Fig. 4 High-expressional SLC10A3 affects the prognosis of CRC patients.A The OS of CRC patients between low and high-expressional groups.B The DSS of CRC patients between low and high-expressional groups.C The PFI of CRC patients between low and high-expressional groups.D-E The survival analysis of SLC10A3 high expression and low expression in COAD and READ based on the TIMER database.F SLC10A3 expression in CRC patients was analyzed based on the tumor metastasis.G-H Nomogram to predict the COAD survival possibility among 1 year, 3 years and 5 years, including age, gender, TNM stage, tumor stage, and risk score.CRC, colorectal cancer; READ, rectal adenocarcinoma; COAD, colon adenocarcinoma.HR hazard ratio, OS overall survival, DSS disease-special survival, PFI progress-free interval

Fig. 5
Fig. 5 Correlation of immune cell infiltration, chemokines and SLC10A3 expression in CRC patients.A Relationships among infiltration levels of 24 immune cell types and SLC10A3 expression profiles by Spearman's analysis in CRC.B-E The infiltration levels of Treg cells, CD8 + cells, NK cells, and T cells in the high-and low-SLC10A3 expression groups in CRC.F-G SLC10A3 expression is related to immune infiltration levels in COAD and READ through TIMER.H The relationship between chemokines and SLC10A3 expression in COAD and READ.CRC colorectal cancer; READ rectal adenocarcinoma, COAD colon adenocarcinoma.*p-value < 0.05; **p-value < 0.01; ***p-value < 0.001

Fig. 6
Fig. 6 Correlation analysis of SLC10A3 and cancer-associated fibroblasts in COAD and READ.A, C The association of SLC10A3 and cancer-associated fibroblast in COAD (p-value = 1.02e−04) and READ (p-value = 5.07e-03) by EPIC.B, D The correlation analysis between the SLC10A3 expression and the expression levels of VIM, ACAT2 in the TCGA dataset.READ rectal adenocarcinoma, COAD colon adenocarcinoma

behavior [ 28 ]
. By secreting diverse cytokines, CAFs establish communication with other stromal cells and tumor cells, resulting in immune cell function inhibition and facilitation of tumor development[29].Cytokines such as TGF-β are secreted by CAFs, leading to DC maturation suppression and Treg differentiation promotion[30][31][32].Consequently, we assessed the correlation between SLC10A3 and CAFs in COAD and READ by utilizing R and TIMER, based on TCGA datasets.Notably, we identified a significant positive correlation between SLC10A3 and CAFs in both COAD (p-value = 1.02e−04) and READ (p-value = 5.07e−03).High expression levels of SLC10A3 were found to be positively associated with CAF markers, including ACAT2 (p-value = 0.008) and VIM (p-value < 0.001).

Fig. 7
Fig. 7 Correlation analysis between SLC10A3 and immune checkpoint factors in CRC.A-F The correlation analysis between the SLC10A3 expression and the expression levels of PMS1, RAD21, DNA2, MLH3, MLH1, PMS2, MSH2, MSH6 and MSH4 in the TCGA-CRC dataset

Fig. 8 Fig. 9
Fig. 8 Screening DEGs and constructing functional analysis between SLC10A3 high-expressional group and SLC10A3 low-expressional group.A Volcano plots of DEGs between the expression of SLC10A3-high and SLC10A3-low in CRC samples.B GO and KEGG analysis among DEGs in CRC as a circle graph.C GSEA analysis of DEGs between SLC10A3-high and SLC10A3-low in CRC samples.CRC colorectal cancer

Table 1
Clinicopathological characteristics of the patient cohorts

Table 2
Univariate and multivariate analyses of SLC10A3 in colorectal adenocarcinoma

Table 3
The relationship of SLC10A3 and immune cell infiltration in colorectal adenocarcinoma

Table 4
The prognostic value of SLC10A3 CpG islands